Efficient base editing with expanded targeting scope using an engineered Spy-mac Cas9 variant
نویسندگان
چکیده
منابع مشابه
Efficient Genome Editing in Apple Using a CRISPR/Cas9 system
Genome editing is a powerful technique for genome modification in molecular research and crop breeding, and has the great advantage of imparting novel desired traits to genetic resources. However, the genome editing of fruit tree plantlets remains to be established. In this study, we describe induction of a targeted gene mutation in the endogenous apple phytoene desaturase (PDS) gene using the ...
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The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system has been widely used for nuclear DNA editing to generate mutations or correct specific disease alleles. Despite its flexible application, it has not been determined if CRISPR/Cas9, originally identified as a bacterial defense system against virus, can be targeted to mitochondria for mtDNA editing. Here, we show t...
متن کاملGenome editing using Cas9 nickases.
The RNA-guided, sequence-specific endonuclease Cas9 has been widely adopted as genome engineering tool due to its efficiency and ease of use. Derived from the microbial CRISPR (clustered regularly interspaced short palindromic repeat) type II adaptive immune system, Cas9 has now been successfully engineered for genome editing applications in a variety of animal and plant species. To reduce pote...
متن کاملTargeted genome and epigenome editing using engineered TALE and CRISPR/Cas9 technologies
The impact of reverse genetics, synthetic biology, and gene therapy has been restricted by the limitations of conventional genetic engineering technologies. To expand the capacity for genetic modification of mammalian cells, we are engineering artificial DNA-binding proteins, including zinc finger proteins, TAL effectors, and CRISPR/Cas9 to regulate and edit endogenous mammalian genes. For exam...
متن کاملPrecise Editing of a Target Base in the Rice Genome Using a Modified CRISPR/Cas9 System.
CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR-associated Cas9) has been widely used in genome editing in a variety of organisms, including rice (Cong et al., 2013; Feng et al., 2013). The majority of the editing events reported in plants typically result in an abundance of random insertions and deletions (Indels) at the target locus through the error-prone non-ho...
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ژورنال
عنوان ژورنال: Cell Discovery
سال: 2019
ISSN: 2056-5968
DOI: 10.1038/s41421-019-0128-4